Translational_Unit

Part:BBa_K3477006:Design

Designed by: Mathilda Johansson   Group: iGEM20_Chalmers-Gothenburg   (2020-10-20)


Gene coding for his-tagged PueA enzyme including BBa_0034 RBS, Bba_J23106 promoter and Bba_0010 term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2030
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal NheI site found at 143
    Illegal SpeI site found at 2031
    Illegal PstI site found at 2045
    Illegal NotI site found at 7
    Illegal NotI site found at 2038
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2031
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 56
    Illegal SpeI site found at 2031
    Illegal PstI site found at 2045
    Illegal NgoMIV site found at 1671
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

The genetic sequence comes from Pseudomonas fluorescens ATCC BAA-477 and was found on Uniprot (https://www.uniprot.org/uniprot/Q4KBS6)

References